• preparation of dna-crosslinked polyacrylamide hydrogels

    Preparation of DNA-crosslinked Polyacrylamide Hydrogels

    2014-08-27· DNA-crosslinked polyacrylamide hydrogels (DNA gels) are dynamic two-dimensional elastic substrates. DNA crosslinks allow for temporal, spatial, and reversible modulation of DNA gel stiffness by addition of single-stranded DNA (ssDNA) to media or buffer 9-11,13,14,18,21 .

  • preparation of dna-crosslinked polyacrylamide hydrogels

    Preparation of DNA-crosslinked Polyacrylamide Hydrogels

    Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.

  • mechanical properties of a reversible, dna-crosslinked

    Mechanical Properties of a Reversible, DNA-Crosslinked

    Mechanical properties of a polyacrylamide gel with reversible DNA crosslinks are presented. In this system, three DNA strands replace traditional chemical crosslinkers. In contrast to thermoset chemically crosslinked polyacrylamide, the new hydrogel is thermoreversible; crosslink dissociation without the addition of heat is also feasible by

  • preparation of dna-crosslinked polyacrylamide hydrogels

    Preparation of DNA-crosslinked Polyacrylamide Hydrogels

    To better address the effects of dynamic stiffness on cell function, we developed a DNA-crosslinked polyacrylamide hydrogel system (DNA gels). Unlike other dynamic substrates, DNA gels have the

  • mechanical properties of a reversible, dna-crosslinked

    Mechanical Properties of a Reversible, DNA-Crosslinked

    Download Citation Mechanical Properties of a Reversible, DNA-Crosslinked Polyacrylamide Hydrogel Mechanical properties of a polyacrylamide gel with reversible DNA crosslinks are presented. In

  • mechanical properties of dna-crosslinked polyacrylamide

    Mechanical Properties of DNA-Crosslinked Polyacrylamide

    Mechanical Properties of DNA-Crosslinked Polyacrylamide Hydrogels with Increasing Crosslinker Density Michelle L. Previtera,1 Uday Chippada,2 Rene S. Schloss,1 Bernard Yurke,3,4 and Noshir A

  • responsive dna-based hydrogels and their applications

    Responsive DNA-based hydrogels and their applications

    Target-responsive hydrogels can be used to precisely control release time and site. Using fluorescent semiconductor QDs as a model molecule, Liedl et al. studied a DNA- triggered release of QDs from a DNA-crosslinked polyacrylamide hydrogel . Addition of the “release” strand of DNA unlinked the gel by removal of the crosslinker strands by

  • mechanical properties of a reversible, dna-crosslinked

    Mechanical properties of a reversible, DNA-crosslinked

    The polymer hydrogels were synthesized based on a reported procedure in synthesis of DNA-crosslinked polyacrylamide hydro- gel ( Lin et al., 2004) with some modifications. The polymeration

  • enzyme-catalysed assembly of dna hydrogel nature

    Enzyme-catalysed assembly of DNA hydrogel Nature

    2006-09-24· The distinct difference of the DNA hydrogel to other bio-inspired hydrogels (including peptide-based, alginate-based and DNA (linear)-polyacrylamide hydrogels 16,17,18,19,20) is that the

  • a portable visual capillary sensor based on functional dna

    A portable visual capillary sensor based on functional DNA

    The analytical performance of the DHCS based on DNA crosslinked hydrogel for the detection of Pb 2+ was investigated by recording the distance and time of different concentrations of Pb 2+ flowing through the capillary tubes. Fig. 3a shows the real-time photographs of DHCS for the detection of Pb 2+.

  • enzyme-catalysed assembly of dna hydrogel nature

    Enzyme-catalysed assembly of DNA hydrogel Nature

    2006-09-24· The distinct difference of the DNA hydrogel to other bio-inspired hydrogels (including peptide-based, alginate-based and DNA (linear)-polyacrylamide hydrogels 16,17,18,19,20) is that the

  • mechanical properties of dna-crosslinked polyacrylamide

    Mechanical Properties of DNA-Crosslinked Polyacrylamide

    Introduction. D NA-crosslinked polyacrylamide hydrogels (DNA gels) are tunable hydrogels developed by our group to mimic the mechanical properties of the dynamic in vivo microenvironment. 1–6 It has been shown that mechanical properties are altered as a function of time via the addition of DNA oligomers. Lin et al. 5 and Previtera et al. 7 demonstrated the ability of DNA gels to compress or

  • characterization of properties of soft hydrogels

    CHARACTERIZATION OF PROPERTIES OF SOFT HYDROGELS

    The present study mainly focuses on characterizing the Young’s modulus (), E shear modulus and Poisson’s ratio (G v) of soft hydrogels using a non-intrusive technique. For this purpose, an apparatus referred to as the “four magnet setup”, which

  • the myth of polyacrylamide hydrogels: polyacrylamide

    The Myth of Polyacrylamide Hydrogels: Polyacrylamide

    Linda Chalker-Scott, Ph.D., Extension Horticulturist and Associate Professor, Puyallup Research and Extension Center, Washington State University The Myth of Polyacrylamide Hydrogels: "Polyacrylamide hydrogels are environmentally safe substances that reduce irrigation needs" The Myth

  • complementary-dna-strand crosslinked polyacrylamide

    Complementary-DNA-strand crosslinked polyacrylamide

    Complementary-DNA-strand crosslinked polyacrylamide hydrogels, Du, Cong, Chemical Engineering, Polyacrylamide hydrogels physically crosslinked by double-stranded DNA have demonstrated potential for drug delivery, biosensing, and tissue engineering due to their programmability, mechanical tunability, molecular-recognition, and temperature reversibility.

  • switchable bifunctional stimuli‐triggered poly‐n

    Switchable Bifunctional Stimuli‐Triggered Poly‐N

    The hydrogels reveal switchable hydrogel‐to‐solution transitions by the reversible crosslinking of the chains at pH 5.2 and the separation of the crosslinking units at pH 7.5, or by the Ag + ion‐stimulated crosslinking of the chains and the reverse dissolution of the hydrogel by the cysteamine‐induced elimination of the Ag + ions. The DNA‐crosslinked hydrogels are thermosensitive and

  • mechanical properties of a reversible, dna-crosslinked

    Mechanical properties of a reversible, DNA-crosslinked

    Mechanical properties of a reversible, DNA-crosslinked polyacrylamide hydrogel. Lin DC(1), Yurke B, Langrana NA. Author information: (1)Department of Mechanical and Aerospace Engineering, Rutgers, State University of New Jersey, 98 Brett Road, Piscataway, NJ 08854, USA.

  • specific dna sequence responisive dna crosslinked hydrogel

    SPECIFIC DNA SEQUENCE RESPONISIVE DNA CROSSLINKED HYDROGEL

    complex structure with hydrogel, Jiayu Liu and Jingkai Guo for their wonderful modeling and simulation work. Also, I want to thank Professor Thao Nguyen and Professor David Gracias for their great guidance on the DNA crosslinked hydrogel project. I want to thank all our current and past labmates, Dr. Abdul Majeed

  • neurite outgrowth on a dna crosslinked hydrogel with

    Neurite Outgrowth on a DNA Crosslinked Hydrogel with

    In this study, a DNA crosslinked hydrogel was employed to examine cellular responses of spinal cord neurons to substrate compliances. Using DNA as crosslinkers in polymeric hydrogel formation has given rise to a new class of hydrogels with a number of attractive properties (e.g., reversible gelation and controlled crosslinking). Here, it was

  • stiffening hydrogels to probe short- and long-term

    Stiffening hydrogels to probe short- and long-term

    2012-04-24· Studying the effects of extracellular matrix stiffening has been impeded because mostin vitromodels are static. Here, dynamic hydrogels are developed that stiffen in

  • polyacrylamide

    Polyacrylamide

    Polyacrylamide (IUPAC poly(2-propenamide) or poly(1-carbamoylethylene), abbreviated as PAM) is a polymer (-CH 2 CHCONH 2-) formed from acrylamide subunits. It can be synthesized as a simple linear-chain structure or cross-linked, typically using N,N '-methylenebisacrylamide.In the cross-linked form, the possibility of the monomer being present is reduced even further.

  • switchable bifunctional stimuli‐triggered poly‐n

    Switchable Bifunctional Stimuli‐Triggered Poly‐N

    The hydrogels reveal switchable hydrogel‐to‐solution transitions by the reversible crosslinking of the chains at pH 5.2 and the separation of the crosslinking units at pH 7.5, or by the Ag + ion‐stimulated crosslinking of the chains and the reverse dissolution of the hydrogel by the cysteamine‐induced elimination of the Ag + ions. The DNA‐crosslinked hydrogels are thermosensitive and

  • cross-linking in hydrogels a review

    Cross-linking in Hydrogels A Review

    Cross-linking in Hydrogels A Review Jaya Maitra*, Vivek Kumar Shukla Gautam Buddha University, Greater Noida, Gautam Budh Nagar-201312 (U.P), India . Abstract . Hydrogels represent a class of high water content polymers with physical or chemical crosslinks. Their physical properties are similar to soft tissues. Cross linking is a

  • stiffening hydrogels to probe short- and long-term

    Stiffening hydrogels to probe short- and long-term

    2012-04-24· Studying the effects of extracellular matrix stiffening has been impeded because mostin vitromodels are static. Here, dynamic hydrogels are developed that stiffen in

  • hydrogel: preparation, characterization, and applications

    Hydrogel: Preparation, characterization, and applications

    The extent of swelling or de-swelling in response to the changes in the external environment of the hydrogel could be so drastic that the phenomenon is referred to as volume collapse or phase transition .Synthetic hydrogels have been a field of extensive research for the past four decades, and it still remains a very active area of research today.

  • mechanical properties of a reversible, dna-crosslinked

    Mechanical Properties of a Reversible, DNA-Crosslinked

    Mechanical properties of a polyacrylamide gel with reversible DNA crosslinks are presented. In this system, three DNA strands replace traditional chemical crosslinkers. In contrast to thermoset chemically-crosslinked polyacrylamide, the new hydrogel is thermoreversible; crosslink dissociation without the addition of heat is also feasible by

  • mechanical properties of dna-crosslinked polyacrylamide

    Mechanical Properties of DNA-Crosslinked Polyacrylamide

    Abstract DNA-cross-linked polyacrylamide hydrogels (DNA gels) are dynamic mechanical substrates. The addition of DNA oligomers can either increase or decrease the crosslinker density to modulate mechanical properties. These DNA-responsive gels show promise as substrates for cell culture and tissue-engineering applications, since the gels allow time-dependent mechanical modulation.

  • dna sequence–directed shape change of photopatterned

    DNA sequence–directed shape change of photopatterned

    2017-09-15· We focused on hydrogels, cross-linked networks of polymers in water, where structural changes can cause extensive expansion or contraction of the material as a whole. To study biomolecular actuation, we considered DNA–cross-linked polyacrylamide hydrogels (Fig. 1A and fig. S1) .

  • neurite outgrowth on a dna crosslinked hydrogel with

    Neurite Outgrowth on a DNA Crosslinked Hydrogel with

    2008-07-11· Mechanical cues arising from extracellular matrices greatly affect cellular properties, and hence, are of significance in designing biomaterials. In this study, a DNA crosslinked hydrogel was employed to examine cellular responses of spinal cord neurons to substrate compliances. Using DNA as crosslinkers in polymeric hydrogel formation has given rise to a new class of hydrogels with a

  • noshir a. langrana rutgers university, biomedical

    Noshir A. Langrana Rutgers University, Biomedical

    Over the past ten years, his group has focused our attention on the design and development of novel dynamic DNA crosslinked hydrogels. It has proved that gel stiffness can be altered dynamically “on-the-fly”. He has shown that Fibroblasts and neurons respond to alterations in substrate rigidity, and the responses vary depending on hydrogel

  • programmable dna hydrogels assembled from multi-domain

    Programmable DNA Hydrogels Assembled from Multi-Domain

    Programmable DNA Hydrogels Assembled from Multi-Domain DNA Strands Huiling Jiang,[a] [Victor Pan, a] [b] Skanda Vivek, Eric R. Weeks[b] and Yonggang Ke*[a] Abstract: Hydrogels are important in biological and medical applications, such as drug delivery and tissue engineering. DNA hydrogels have attracted significant attention due to

  • measurement of young’s modulus of polyacrylamide gel

    Measurement of Young’s Modulus of Polyacrylamide Gel

    Measurement of Young’s Modulus of Polyacrylamide Gel by Digital Holography Xiao Yu, Changgeng Liu, David C.Clark, and Myung K. Kim Digital Holography and Microscopy Laboratory, Department of Physics, University of South Florida, 4202 E. Fowler Avenue,

  • integration of switchable dna-based hydrogels with

    Integration of Switchable DNA-Based Hydrogels with

    A novel method to assemble acrylamide/acrydite DNA copolymer hydrogels on surfaces, specifically gold-coated surfaces, is introduced. The method involves the synthesis of two different copolymer chains consisting of hairpin A, HA, modified acrylamide copolymer and hairpin B, HB, acrylamide copolymer. In the presence of a nucleic acid promoter monolayer associated with the surface, the

  • received new jersey

    RECEIVED New Jersey

    Introduction of a DNA crosslinking mechanism to polymer gel formation gives rise to many new possibilities, such as modifying DNA gels in a controlled temporal and spatial manner. Meanwhile, due to the similarity between bis- and DNA-crosslinked polyacrylamide hydrogels (Bis- and DNA-gel), neuronal cell culture on bis-gels could provide general

  • stimuli-responsive nucleic acid-based polyacrylamide

    Stimuli-Responsive Nucleic Acid-Based Polyacrylamide

    hydrogel coating is degraded via the formation of the ATP–aptamer complex, resulting in the release of doxorubicin drug. In addition to the introduction of a general means to synthesize drug-loaded stimuli-responsive nucleic acid-based polyacrylamide hydrogel-coated NMOFs hybrids, the functionalized

  • gel electrophoresis of proteins

    Gel electrophoresis of proteins

    Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium: SDS polyacrylamide gel electrophoresis (in short: gel electrophoresis, PAGE, or SDS-electrophoresis), free-flow electrophoresis, electrofocusing, isotachophoresis

  • ph-indicating colorimetric hydrogel for wound dressing

    pH-Indicating Colorimetric Hydrogel for Wound Dressing

    the wound, a novel smart hydrogel wound dressing incorporating modified pH indicator dyes was developed. Phenol red (PR) was used as the dye and successfully modified with methacrylate to allow a copolymerization with the alginate/Polyacrylamide (PAAm) hydrogel matrix. This covalent attachment prevented the dye from leaching out of the matrix. The

  • miracle, mythor marketing

    miracle, mythor marketing

    recommended that polyacrylamide gels used in cosmetics contain a residual monomer level of only 0.1 to 0.5 ppm. Therefore, the PAM hydrogels manufactured for agricultural and garden use can contain much greater concentrations of toxic acrylamide than that found in personal products. While new PAM hydrogels contain

  • sds-page to determine the molecular weight

    SDS-PAGE to Determine the Molecular Weight

    2006-06-16· The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (Weber, K., and Osborn, M. (1969) J. Biol. Chem. 244, 4406–4412) This Journal of Biological Chemistry (JBC) Classic on using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to determine the molecular weight of proteins is one of our most

  • noshir a. langrana rutgers university, mechanical

    Noshir A. Langrana Rutgers University, Mechanical

    Over the past ten years, his group has focused our attention on the design and development of novel dynamic DNA crosslinked hydrogels. It has proved that gel stiffness can be altered dynamically “on-the-fly”. He has shown that Fibroblasts and neurons respond to alterations in substrate rigidity, and the responses vary depending on hydrogel

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