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    Polyacrylamide, PAM, anionic polyacrylamide. Polyacrylamide(PAM) is applicable when the pH value of the handled water is from neutral to alkaline and the water is a kind of suspension containing more inorganic material. Polyacrylamide supplier distributor- CAS 9003-05-8

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    2019-04-01· The LNCaP cells were collected and the total protein was extracted by lysing with cell lysis buffer (Beyotime, Haimen, China). The proteins (30 μg per lane) were separated on 12% SDS-polyacrylamide gels and subsequently transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). The primary antibodies to pro

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    polyacrylamide [106] and PVP [122]. However, the COF did not decrease further when water content was increased above 80% in PAAc hydrogels sliding against glass [187]. Similarly, wear decreased by raising the water content of pHEMA hydrogels from 65% to 75% [188], but increased in PVA/PVP hydrogels when water content increased from 85% to 90%

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    Protein concentrations were determined using a BCA protein assay kit (Beyotime, China). Equal amounts of protein were separated on 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride membrane (PVDF; Millipore, Billerica, MA, USA). The membranes were blocked for 1 h with 5% fat-free milk at RT

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    MicroRNA-206 regulates the secretion of inflammatory

    2016-08-19· Human THP-1 macrophages were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and grown in RPMI-1640 (Abcam, Cambridge, MA) media supplemented with 10% fetal bovine serum (FBS, Gibco, Rockville, MD) and 1% pen-strep at 37 °C in a humidified atmosphere with 5% CO 2.

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    LncRNA MALAT1 promotes osteoarthritis by modulating miR

    Dist.,Chongqing 400038,People’sRepublicof China. Zhang et al. Cell Biosci Page 2 of 12 D1 expression by sponging miR-206, resulting in regu-lating chondrocytes proliferation in O[18]ncRNA PVT1 could sponge for miR-488-3p and regulate chon-drocyte apoptosis in OA [19]ncRNA small nucleolar RNA ho(NH)omotes the proliferation of chondrocytes via miR-26a/SOX2 signal axis in OA [20] LncRNA-CIR

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    The satellite glial cells (SGCs) of the dorsal root ganglia (DRG) expressed P2X4 receptor. In this study, we investigated the abnormal sympathetic activity after myocardial ischemia (MI) involving P2X4 receptor in the cervical DRG SGC.

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    Abnormal sympathetic activity after myocardial ischemia

    The satellite glial cells (SGCs) of the dorsal root ganglia (DRG) expressed P2X4 receptor. In this study, we investigated the abnormal sympathetic activity after myocardial ischemia (MI) involving P2X4 receptor in the cervical DRG SGC.

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    MicroRNA-16 is putatively involved in the NF-κB pathway

    Protein concentrations were determined using a BCA protein assay kit (Beyotime, China). Equal amounts of protein were separated on 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride membrane (PVDF; Millipore, Billerica, MA, USA). The membranes were blocked for 1 h with 5% fat-free milk at RT

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    MicroRNA-206 regulates the secretion of inflammatory

    2016-08-19· Human THP-1 macrophages were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and grown in RPMI-1640 (Abcam, Cambridge, MA) media supplemented with 10% fetal bovine serum (FBS, Gibco, Rockville, MD) and 1% pen-strep at 37 °C in a humidified atmosphere with 5% CO 2.

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    Long noncoding RNA HAGLROS promotes cell proliferation

    Following different transfections, cells (Huh7 or HepG2.2.15) were obtained by centrifugation and were lysed with a cell lysis buffer (Sangon Biotech) for collecting total proteins. The proteins (50 μg/lane) were separated onto 12% SDS-polyacrylamide gels, and, subsequently, were transferred onto polyvinylidene fluoride membranes (Millipore

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  • microrna-16 is putatively involved in the nf-κb pathway

    MicroRNA-16 is putatively involved in the NF-κB pathway

    MicroRNAs (miRNAs) act as important post-transcriptional regulators of gene expression by targeting the 3′-untranslated region of their target genes. Altered expression of miR-16 is reported in

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    MicroRNA-16 is putatively involved in the NF-κB pathway

    Protein concentrations were determined using a BCA protein assay kit (Beyotime, China). Equal amounts of protein were separated on 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride membrane (PVDF; Millipore, Billerica, MA, USA).

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    The aim of this study was to evaluate the performance, nutrient utilisation and energy metabolism of broiler chicks fed eight different wheat samples, supplemented or not with xylanase.

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    Bei Jing, China e-mail: [email protected] 123 Med Oncol (2014) 31:982 DOI 10.1007/s12032-014-0982-8. 3 0-untranslated region (3 -UTR) in target mRNAs [3]. Growing evidence suggests that miRNAs play an important role in various biological processes including cell prolif-eration, development, and differentiation. Furthermore, the dysregulation of miRNAs is common in various carcino-mas

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    (PDF) Long noncoding RNA SNHG15 enhances the

    Colon cancer, also known as colorectal carcinoma (CRC), remains to be one of the most mainsprings of cancer-produced deaths entire world. We planned to grab the role and possible biological cause

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    MicroRNA-206 regulates the secretion of inflammatory

    T D ACCEPTED MANUSCRIPT MicroRNA-206 regulates the secretion of inflammatory cytokines and MMP9 expression by targeting TIMP3 in Mycobacterium tuberculosis–infected THP-1 human

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    Phosphatase Actin Regulator-1 (PHACTR-1) Knockdown Suppresses Cell Proliferation and Migration and Promotes Cell Apoptosis in the bEnd.3 Mouse Brain Capillary Endothelial Cell Line

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    miR-150 promotes human breast cancer growth Europe

    Figure S2: miR-150 promotes the cell growth and inhibits the cell apoptosis of breast cancer cells. (A) Bars represent the mean of total number of colonies ± SD of three independent experiments. (B) Percentage of BrdU + cells determined by flow cytometric analysis of BrdU immunostaining in MCF-7 or MDA-MB-231 cells transfected with miR-150 mimics or miR-150 inhibitors.

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  • upregulation of lncrna haglros enhances the

    Upregulation of lncRNA HAGLROS enhances the

    We lysed cells using cell lysis buffer (Beyotime, Haimen, China) for extraction of total protein. The total isolated proteins (30 μg per lane) were separated on 12% SDS-polyacrylamide gels and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Primary antibodies to β-actin, ATG14, apoptotic proteins

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    Long noncoding RNA SNHG15 enhances the development

    In China, there is an observation of a very high incidence and mortality of CRC, which are increasing yearly [3 Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66: 115 132.,,[Web of Science ®],[Google Scholar]].

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